Insight into the First Draft Genome Sequence of the Genus Sediminibacillus, Sediminibacillus halophilus Strain NSP9.3

We report the 3.98-Mbp first draft genome sequence of Sediminibacillus halophilus strain NSP9.3, a moderate halophile isolated from a seasonal salt marsh of the Great Rann of Kutch, India. Exploring the genome of this organism will facilitate the understanding of the mechanism(s) of osmotolerance and survival in differential osmolarity.

in the saline environments of the Rann of Kutch, India, were sequenced to understand the mechanism(s) of osmotolerance (1)(2)(3)(4). Sediminibacillus halophilus strain NSP9.3 (16S rRNA, GenBank accession no. JX518263), a moderately halophilic bacterium, was isolated from a seasonal salt marsh of the Great Rann of Kutch, India. This bacterium grows optimally at a 5% NaCl (range, 0 to 20%) concentration in growth medium at 37°C and 7.5 pH. The genome of Sediminibacillus halophilus strain NSP9.3 was sequenced to gain insight into its osmotolerance mechanism(s).
De novo assembly of reads using GS De Novo Assembler v 2.6 (5) gave approximately 134-fold coverage, with 10 scaffolds of 3,985,996 bp and 25 contigs of 3,983,638 bp. N 50 scaffold lengths of 693,230 bp (smallest, 2,877 bp, and largest, 1,699,278 bp) and N 50 contig lengths of 276,706 bp (smallest, 2,877 bp, and largest, 572,364 bp) were obtained. All assembly data were deposited in the DDBJ/EMBL/GenBank nucleotide sequence database.
We are further exploring the first draft genome of Sediminibacillus halophilus NSP9.3 to understand its survival and osmoadaptation strategies.
Nucleotide sequence accession numbers. This whole-genome shotgun project has been deposited at DDBJ/EMBL/GenBank under the accession number AWXX00000000. The version described in this paper is version AWXX01000000.

ACKNOWLEDGMENTS
The work was carried out in the subproject "Diversity analysis of Bacillus and other predominant genera in extreme environments and its utilization in agriculture" of the National Agricultural Innovation Project (NAIP) and "Exploring bacterial and archaebacterial diversity in Kutch eco-region of Gujarat for agricultural and industrial applications" under the Application of Microorganisms in Agriculture and Allied Sectors (AMAAS) of the Indian Council of Agricultural Research (ICAR). We thank ICAR for funding through NAIP and AMAAS.
We thank the directors and coordinators, NAIP, and Directorate of Groundnut Research, Junagadh, and NBAIM, Mau, for help during the course of this study. We also thank Macrogen, Inc., South Korea, and Sequencher Tech Pvt. Ltd., Ahmedabad, India, for the genomic services provided.